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phix control  (Illumina Inc)


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    Structured Review

    Illumina Inc phix control
    Phix Control, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phix+control/PhiX+Control+v3/pmc13652054-46-7-11
    Average 99 stars, based on 3854 article reviews
    phix control - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Control:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: .. Following purification, libraries were sequenced with 25% PhiX control on an Illumina NovaSeq 6000 using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles). ..

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Article Title: Thiamine cross-feeding drives microbial coexistence in the leaf microbiome.
    Article Snippet: .. Libraries were prepared with the MiSeq Reagent Kit v.3 for 2 × 300 bp paired-end reads (Illumina) with 3% PhiX control. .. Quality assessment and the eventual presence of contaminants were assessed using a bioanalyzer (Agilent).

    Article Title: Universal donor cells
    Article Snippet: Targeted RNAseq for gene expression analysis was performed using Illumina TruSeq and a custom panel of oligos targeting 111 genes. .. The libraries were subsequently diluted to a concentration of 4 nM and pooled, followed by denaturing, spike in of PhiX control, and further dilution to 10-12 pM prior to loading on the Illumina MiSeq sequencer. .. At end of each differentiation stage, 10 μL APV (aggregated pellet volume) was collected and extracted using the Qiagen RNeasy or RNeasy 96 spin column protocol, including on-column DNase treatment.

    Article Title: Single-cell profiling uncovers a rare mesenchymal-like urothelial state associated with sarcomatoid bladder cancer
    Article Snippet: The final libraries were assessed using D1000 screentape on a TapeStation 4200 (Agilent Technologies) and quantified using the KAPA qPCR quantification kit for Illumina platforms (Kapa Biosystems). .. Libraries were pooled, denatured, and diluted to 300 pM with 1% PhiX control, then loaded onto a NovaSeq Reagent cartridge and sequenced on the NovaSeq 6000 system according to Illumina’s recommended protocol by the RPCCC Genomics Shared Resource. ..

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection
    Article Snippet: Index PCR was performed using the Nextera XT Index Kit v2 Set B and 2 × KAPA HiFi HotStart ReadyMix, and the libraries were quantified using a Qubit 4 Fluorometer with the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). .. The libraries were denatured with NaOH, diluted, mixed with 5% PhiX control, and sequenced on a MiSeq i100 plus platform (Illumina, USA) using a 2 × 300 cycle V3 kit. ..

    Article Title: Intermittent low-strength ultrasonication enhances methane production by stimulating direct interspecies electron transfer in microbial electrolysis cell–anaerobic digestion systems
    Article Snippet: This study investigated the effects of intermittent low-strength ultrasonication (LS-ultrasonication) on the performance of a microbial electrolysis cell–anaerobic digestion (MEC–AD) system.. However, the effect of ultrasonication interval in MEC–AD systems remains unclear.. Ultrasonication was intermittently applied at 0.005 W mL 1 with pulse intervals of 30, 60, and 120 s. Methane production in the ultrasonication-assisted MEC systems increased by 16.9–40.9% compared with the control MEC.

    Concentration Assay:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Universal donor cells
    Article Snippet: Targeted RNAseq for gene expression analysis was performed using Illumina TruSeq and a custom panel of oligos targeting 111 genes. .. The libraries were subsequently diluted to a concentration of 4 nM and pooled, followed by denaturing, spike in of PhiX control, and further dilution to 10-12 pM prior to loading on the Illumina MiSeq sequencer. .. At end of each differentiation stage, 10 μL APV (aggregated pellet volume) was collected and extracted using the Qiagen RNeasy or RNeasy 96 spin column protocol, including on-column DNase treatment.

    Purification:

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: .. Following purification, libraries were sequenced with 25% PhiX control on an Illumina NovaSeq 6000 using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles). ..

    Article Title: Intermittent low-strength ultrasonication enhances methane production by stimulating direct interspecies electron transfer in microbial electrolysis cell–anaerobic digestion systems
    Article Snippet: This study investigated the effects of intermittent low-strength ultrasonication (LS-ultrasonication) on the performance of a microbial electrolysis cell–anaerobic digestion (MEC–AD) system.. However, the effect of ultrasonication interval in MEC–AD systems remains unclear.. Ultrasonication was intermittently applied at 0.005 W mL 1 with pulse intervals of 30, 60, and 120 s. Methane production in the ultrasonication-assisted MEC systems increased by 16.9–40.9% compared with the control MEC.



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