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phix control  (Illumina Inc)


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    Structured Review

    Illumina Inc phix control
    Phix Control, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phix+control/PhiX+Control+v3/pmc13652054-46-7-11
    Average 99 stars, based on 3837 article reviews
    phix control - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Control:

    Article Title: Effects of selection on production parameters and intestinal microbiota in heritage and modern broiler chickens
    Article Snippet: The pooled (96 barcoded amplicons) DNA library (4 nmol/L) and PhiX control v3 (Illumina, Inc., 4 nmol/L) were denatured with 0.2 mol/L NaOH (Sigma-Aldrich, Corp., St. Louis, MO, USA) and diluted to a final concentration of 4 pmol/L. .. The library was mixed with PhiX control (20% v/v) and pair-end 2 × 300-bp sequencing was performed using the Illumina MiSeq platform and a MiSeq Reagent Kit v3 (Illumina). ..

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: .. Following purification, libraries were sequenced with 25% PhiX control on an Illumina NovaSeq 6000 using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles). ..

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Article Title: Diel and eddy driven changes in microbial gene expression and biogeochemistry in the oceanic chlorophyll maximum.
    Article Snippet: .. A phiX control (Illumina) was added to an estimated final contribution of 5% of the total estimated sequence depth. .. DNA was sequenced as 150 bp paired end reads on an Illumina NextSeq 500 using a V2 high output 300 cycle reagent kit.

    Article Title: Universal donor cells
    Article Snippet: Targeted RNAseq for gene expression analysis was performed using Illumina TruSeq and a custom panel of oligos targeting 111 genes. .. The libraries were subsequently diluted to a concentration of 4 nM and pooled, followed by denaturing, spike in of PhiX control, and further dilution to 10-12 pM prior to loading on the Illumina MiSeq sequencer. .. At end of each differentiation stage, 10 μL APV (aggregated pellet volume) was collected and extracted using the Qiagen RNeasy or RNeasy 96 spin column protocol, including on-column DNase treatment.

    Article Title: Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
    Article Snippet: The PCR product was directly used for library preparation using the KAPA HyperPlus Library Preparation Kit (Kapa Biosystems, product #KK8514). .. To add diversity in the library, the samples were spiked with 15% PhiX Control purchased from Illumina, Inc. (product #FC-110-3001). .. The library was then loaded onto Illumina's 600 cycle MiSeq Reagent Kit v3 (Illumina, product #MS-102-3003) and run on the MiSeq instrument.

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection
    Article Snippet: Index PCR was performed using the Nextera XT Index Kit v2 Set B and 2 × KAPA HiFi HotStart ReadyMix, and the libraries were quantified using a Qubit 4 Fluorometer with the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). .. The libraries were denatured with NaOH, diluted, mixed with 5% PhiX control, and sequenced on a MiSeq i100 plus platform (Illumina, USA) using a 2 × 300 cycle V3 kit. ..

    Sequencing:

    Article Title: Effects of selection on production parameters and intestinal microbiota in heritage and modern broiler chickens
    Article Snippet: The pooled (96 barcoded amplicons) DNA library (4 nmol/L) and PhiX control v3 (Illumina, Inc., 4 nmol/L) were denatured with 0.2 mol/L NaOH (Sigma-Aldrich, Corp., St. Louis, MO, USA) and diluted to a final concentration of 4 pmol/L. .. The library was mixed with PhiX control (20% v/v) and pair-end 2 × 300-bp sequencing was performed using the Illumina MiSeq platform and a MiSeq Reagent Kit v3 (Illumina). ..

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Article Title: Diel and eddy driven changes in microbial gene expression and biogeochemistry in the oceanic chlorophyll maximum.
    Article Snippet: .. A phiX control (Illumina) was added to an estimated final contribution of 5% of the total estimated sequence depth. .. DNA was sequenced as 150 bp paired end reads on an Illumina NextSeq 500 using a V2 high output 300 cycle reagent kit.

    Concentration Assay:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Universal donor cells
    Article Snippet: Targeted RNAseq for gene expression analysis was performed using Illumina TruSeq and a custom panel of oligos targeting 111 genes. .. The libraries were subsequently diluted to a concentration of 4 nM and pooled, followed by denaturing, spike in of PhiX control, and further dilution to 10-12 pM prior to loading on the Illumina MiSeq sequencer. .. At end of each differentiation stage, 10 μL APV (aggregated pellet volume) was collected and extracted using the Qiagen RNeasy or RNeasy 96 spin column protocol, including on-column DNase treatment.

    Purification:

    Article Title: Gut microbial metabolism of peanuts generates immunoregulatory metabolites
    Article Snippet: .. Following purification, libraries were sequenced with 25% PhiX control on an Illumina NovaSeq 6000 using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles). ..



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